Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
نویسندگان
چکیده
منابع مشابه
Chip-based three-dimensional cell culture in perfused micro-bioreactors.
We have developed a chip-based cell culture system for the three-dimensional cultivation of cells. The chip is typically manufactured from non-biodegradable polymers, e.g., polycarbonate or polymethyl methacrylate by micro injection molding, micro hot embossing or micro thermo-forming. But, it can also be manufactured from bio-degradable polymers. Its overall dimensions are 0.7 1 x 20 x 20 x 0....
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Amplified neuronal signals can be recorded by microelectrode array (MEA) in microfluidic channels where neurons were cultured in 3D. MEA was located at the bottom of channels where only axons, isolated from somas by collagen blocks, were grown. The impedance of microchannels is higher than that of open chambers, creating enhanced signals at electrodes compared to simple chambers. The first sign...
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Cell culture is an essential tool in drug discovery, tissue engineering and stem cell research. Conventional tissue culture produces two-dimensional cell growth with gene expression, signalling and morphology that can be different from those found in vivo, and this compromises its clinical relevance. Here, we report a three-dimensional tissue culture based on magnetic levitation of cells in the...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2008
ISSN: 1940-087X
DOI: 10.3791/564